Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, NTERA-2 cl.D1 cells, rat testis tissue, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26993-1-AP targets NKAPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25779 Product name: Recombinant human NKAPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-275 aa of BC101685 Sequence: YSDSDSNSESDTNSDSDDDKKRVKAKKKKKKKKHKTKKKKNKKTKKESSDSSCKDSEEDLSEATWMEQPNVADTM 相同性解析による交差性が予測される生物種 |
| Full Name | NFKB activating protein-like |
| Observed molecular weight | 52 kDa, 65 kDa |
| GenBank accession number | BC101685 |
| Gene Symbol | NKAPL |
| Gene ID (NCBI) | 222698 |
| RRID | AB_3742208 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5M9Q1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NKAPL (NF-kappa-B activating protein-like), also known as C6orf194, is a nuclear protein-coding gene located on chromosome 6p22.1. Initially identified as a germ cell-specific suppressor of Notch signaling essential for spermatogenesis, NKAPL has recently emerged as a tumor suppressor in non-small cell lung cancer through TRIM21 stabilization and NF-κB pathway inhibition. Additionally, NKAPL promoter methylation serves as a prognostic biomarker in multiple malignancies.(PMID: 25875095; 40605974)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NKAPL antibody 26993-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |