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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

NIT1 Polyclonal antibody

NIT1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14380-1-AP

Synonyms

NIT1, nitrilase 1, Nitrilase homolog 1

Validation Data Gallery

Applications:
  • WB analysis using 14380-1-AP

    WB analysis using 14380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 14380-1-AP

    WB analysis of HEK-293T using 14380-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14380-1-AP

    WB analysis of HeLa using 14380-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 14380-1-AP

    WB analysis of HepG2 using 14380-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14380-1-AP

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14380-1-AP

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14380-1-AP

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas cancer using 14380-1-AP

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 14380-1-AP

    IF Staining of HepG2 using 14380-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14380-1-AP targets NIT1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIT1 fusion protein Ag5764 相同性解析による交差性が予測される生物種
Full Name nitrilase 1
Calculated molecular weight 40 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC046149
Gene symbol NIT1
Gene ID (NCBI) 4817
RRIDAB_2151314
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NIT1 antibody 14380-1-APDownload protocol
IHC protocol for NIT1 antibody 14380-1-APDownload protocol
IF protocol for NIT1 antibody 14380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

NIT1 suppresses tumour proliferation by activating the TGFβ1-Smad2/3 signalling pathway in colorectal cancer.

Authors - Chun Lin
  • KD Validated
View Article