Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27077-1-AP targets NIPA1 in WB, ELISA applications and shows reactivity with human, Mouse, Rat samples.
| Tested Reactivity | human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23847 Product name: Recombinant human NIPA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 194-277 aa of BC025678 Sequence: RYINKALECFDSSVFGAIYYVVFTTLVLLASAILFREWSNVGLVDFLGMACGFTTVSVGIVLIQVFKEFNFNLGEMNKSNMKTD 相同性解析による交差性が予測される生物種 |
| Full Name | non imprinted in Prader-Willi/Angelman syndrome 1 |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC025678 |
| Gene Symbol | NIPA1 |
| Gene ID (NCBI) | 123606 |
| RRID | AB_3085923 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7RTP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nonimprinted in Prader-Willi/Angelman loci 1 (NIPA1, also known as SPG6) gene is located in the pericentromeric region of chromosome 15q and encodes a magnesium transporter located in the plasma membrane and early endosomes, implicated in neuronal development and maintenance (PMID: 17166836). NIPA1 is highly conserved along phylogeny and highly expressed in neuronal tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NIPA1 antibody 27077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |