Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16830-1-AP targets NIP30 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10439 Product name: Recombinant human NIP30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-254 aa of BC071952 Sequence: MDGGDDGNLIIKKRFVSEAELDERRKRRQEEWEKVRKPEDPEECPEEVYDPRSLYERLQEQKDRKQQEYEEQFKFKNMVRGLDEDETNFLDEVSRQQELIEKQRREEELKELKEYRNNLKKVGISQENKKEVEKKLTVKPIETKNKFSQAKLLAGAVKHKSSESGNSVKRLKPDPEPDDKNQEPSSCKSLGNTSLSGPSIHCPSAAVCIGILPGLGAYSGSSDSESSSDSEGTINATGKIVSSIFRTNTFLEAP 相同性解析による交差性が予測される生物種 |
| Full Name | NEFA-interacting nuclear protein NIP30 |
| Calculated molecular weight | 254 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC071952 |
| Gene Symbol | NIP30 |
| Gene ID (NCBI) | 80011 |
| RRID | AB_2109703 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZU8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NIP30, also known as NEFA-interacting nuclear protein 30, FAM192A (family with sequence similarity 192, member A), is a 254 amino acid nuclear protein encoded by a gene that maps to human chromosome 16q13.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NIP30 antibody 16830-1-AP | Download protocol |
| IP protocol for NIP30 antibody 16830-1-AP | Download protocol |
| WB protocol for NIP30 antibody 16830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells. | ||
Retrovirology Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses. |
