Validation Data Gallery
Tested Applications
| Positive WB detected in | HuT 78 cells, Karpas-299 cells, MOLT-4 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
14085-1-AP targets NINJ2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5215 Product name: Recombinant human NINJ2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-158 aa of BC057766 Sequence: MESARENIDLQPGSSDPRSQPINLNHYATKKSVAESMLDVALFMSNAMRLKAVLEQGPSSHYYTTLVTLISLSLLLQVVIGVLLVVIARLNLNEVEKQWRLNQLNNAATILVFFTVVINVFITAFGAHKTGFLAARASRNPL 相同性解析による交差性が予測される生物種 |
| Full Name | ninjurin 2 |
| Calculated molecular weight | 142 aa, 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC057766 |
| Gene Symbol | NINJ2 |
| Gene ID (NCBI) | 4815 |
| RRID | AB_10642559 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZG7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NINJ2 antibody 14085-1-AP | Download protocol |
| WB protocol for NINJ2 antibody 14085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cells Development of Novel Peptides That Target the Ninjurin 1 and 2 Pathways to Inhibit Cell Growth and Survival via p53 | ||
Cell Commun Signal Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulation. |