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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIFK Polyclonal antibody (12615-1-AP)

    WB analysis using 12615-1-AP (same clone as 12615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12615-1-AP (NIFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NIFK Polyclonal antibody (12615-1-AP)

    WB analysis of HEK-293 using 12615-1-AP (same clone as 12615-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12615-1-AP (MKI67IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human liver cancer using 12615-1-AP (same clone as 12615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human liver cancer using 12615-1-AP (same clone as 12615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human renal cell carcinoma using 12615-1-AP (same clone as 12615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human renal cell carcinoma using 12615-1-AP (same clone as 12615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NIFK Polyclonal antibody (12615-1-AP)

    IF Staining of MCF-7 using 12615-1-AP (same clone as 12615-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 12615-1-AP (NIFK antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using NIFK Polyclonal antibody (12615-1-AP)

    FC experiment of MCF-7 using 12615-1-AP (same clone as 12615-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug NIFK Polyclonal antibody (12615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12615-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12615-1-PBS targets NIFK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIFK fusion protein Ag3284 相同性解析による交差性が予測される生物種
Full Name MKI67 (FHA domain) interacting nucleolar phosphoprotein
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC024238
Gene Symbol NIFK
Gene ID (NCBI) 84365
RRIDAB_2142384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYG3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.