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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NIF3L1 Polyclonal antibody (10549-1-AP)

    WB analysis of HepG2 using 10549-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NIF3L1 Polyclonal antibody (10549-1-AP)

    WB analysis using 10549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NIF3L1 Polyclonal antibody (10549-1-AP)

    WB analysis of HEK-293 using 10549-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NIF3L1 Polyclonal antibody (10549-1-AP)

    WB analysis of PC-3 using 10549-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NIF3L1 Polyclonal antibody

NIF3L1 Polyclonal Antibody for WB, ELISA
Cat No. 10549-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

ALS2CR1, CALS 7, MDS015, My018, NIF3 like protein 1, NIF3L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, PC-3 cells, PC-12 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10549-1-AP targets NIF3L1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0819

Product name: Recombinant human NIF3L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-377 aa of BC007654

Sequence: MLSSCVRPVPTTVRFVDSLICNSSRSFMDLKALLSSLNDFASLSFAESWDNVGLLVEPSPPHTVNTLFLTNDLTEEVMEEVLQKKADLILSYHPPIFRPMKRITWNTWKERLVIRALENRVGIYSPHTAYDAAPQGVNNWLAKGLGACTSRPIHPSKAPNYPTEGNHRVEFNVNYTQDLDKVMSAVKGIDGVSVTSFSARTGNEEQTRINLNCTQKALMQVVDFLSRNKQLYQKTEILSLEKPLLLHTGMGRLCTLDESVSLATMIDRIKRHLKLSHIRLALGVGRTLESQVKVVALCAGSGSSVLQGVEADLYLTGEMSHHDTLDAASQGINVILCEHSNTERGFLSDLRDMLDSHLENKINIILSETDRDPLQVV

相同性解析による交差性が予測される生物種
Full Name NIF3 NGG1 interacting factor 3-like 1 (S. pombe)
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa, 39 kDa
GenBank accession numberBC007654
Gene Symbol NIF3L1
Gene ID (NCBI) 60491
RRIDAB_2153439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NIF3L1 antibody 10549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols