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Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using NICN1 Monoclonal antibody (67328-1-Ig)

    WB analysis of U-251 using 67328-1-Ig (same clone as 67328-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 67328-1-Ig (NICN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using NICN1 Monoclonal antibody (67328-1-Ig)

    WB analysis of human testis using 67328-1-Ig (same clone as 67328-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67328-1-Ig (NICN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67328-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using NICN1 Monoclonal antibody (67328-1-Ig)

    WB analysis of NCCIT using 67328-1-Ig (same clone as 67328-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67328-1-Ig (NICN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67328-1-PBS in a different storage buffer formulation.

NICN1 Monoclonal antibody, PBS Only

NICN1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67328-1-PBS
Clone No.5B7G6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

NICN1, nicolin 1, PGs5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67328-1-PBS targets NICN1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6581

Product name: Recombinant human NICN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-213 aa of BC050653

Sequence: MSRVLVPCHVKGSVALQVGDVRTSQGRPGVLVIDVTFPSVAPFELQEITFKNYYTAFLSIRVRQYTSAHTPAKWVTCLRDYCLMPDPHSEEGAQEYVSLFKHQMLCDMARISELRLILRQPSPLWLSFTVEELQIYQQGPKSPSVTFPKWLSHPVPCEQPALLREGLPDPSRVSSEVQQMWALTEMIRASHTSARIGRFDVDGCYDLNLLSYT

相同性解析による交差性が予測される生物種
Full Name nicolin 1
Calculated molecular weight 24 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC050653
Gene Symbol NICN1
Gene ID (NCBI) 84276
RRIDAB_2882587
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BSH3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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