Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells |
| Positive IHC detected in | human heart tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21310-1-AP targets NHLRC1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15940 Product name: Recombinant human NHLRC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 86-395 aa of BC103888 Sequence: VLHLIELLGSALRQSPAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWG 相同性解析による交差性が予測される生物種 |
| Full Name | NHL repeat containing 1 |
| Calculated molecular weight | 395 aa, 42 kDa |
| Observed molecular weight | 45-47 kDa |
| GenBank accession number | BC103888 |
| Gene Symbol | NHLRC1 |
| Gene ID (NCBI) | 378884 |
| RRID | AB_10733882 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6VVB1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NHLRC1(NHL repeat-containing protein 1) is also named as Malin,EPM2B. It is predicted to encode a 42.3-kDa (395amino acid) protein, malin, containing a detectable zinc-binding RING finger and six NHL-repeat domains(PMID:12958597).It can suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS).Defects in NHLRC1 are a cause of progressive myoclonic epilepsy type 2 (EPM2).This antibody is specific to NHLRC1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NHLRC1 antibody 21310-1-AP | Download protocol |
| WB protocol for NHLRC1 antibody 21310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |