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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using NHE3 Polyclonal antibody (27190-1-AP)

    WB analysis of mouse small intestine using 27190-1-AP (same clone as 27190-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP (NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using NHE3 Polyclonal antibody (27190-1-AP)

    IP experiment of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NHE3 Polyclonal antibody (27190-1-AP)

    IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). Arrows show brush border. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NHE3 Polyclonal antibody (27190-1-AP)

    IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

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NHE3 Polyclonal antibody, PBS Only

NHE3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27190-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SLC9A3, NHE-3

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27190-1-PBS targets NHE3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHE3 fusion protein Ag25637 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3
Calculated molecular weight 834 aa, 93 kDa
Observed molecular weight 80-100 kDa
GenBank accession numberBC101669
Gene Symbol NHE3
Gene ID (NCBI) 6550
RRIDAB_2880793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48764
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).