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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NGFR/TNFRSF16 Polyclonal antibody (32505-1-AP)

    WB analysis using 32505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32505-1-AP (NGFR/TNFRSF16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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NGFR/TNFRSF16 Polyclonal antibody

NGFR/TNFRSF16 Polyclonal Antibody for WB, ELISA
Cat No. 32505-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CD271, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32505-1-AP targets NGFR/TNFRSF16 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name nerve growth factor receptor (TNFR superfamily, member 16)
Calculated molecular weight46 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_033217.3
Gene Symbol Ngfr
Gene ID (NCBI) 18053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9Z0W1-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Protocols

Product Specific Protocols
WB protocol for NGFR/TNFRSF16 antibody 32505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols