Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, SW480 cells, HT-29 cells, RD cells, LN-229 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86920-2-RR targets NGFR/TNFRSF16 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4829 Product name: Recombinant Human NGFR/TNFRSF16 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 29-250 aa of NM_002507.4 Sequence: KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDN 相同性解析による交差性が予測される生物種 |
| Full Name | nerve growth factor receptor (TNFR superfamily, member 16) |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | NM_002507.4 |
| Gene Symbol | p75NTR |
| Gene ID (NCBI) | 4804 |
| RRID | AB_3745205 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08138-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
p75NTR, also named TNFRSF16, Gp80-LNGFR, p75 ICD, and CD271, is a low-affinity receptor that can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NGFR/TNFRSF16 antibody 86920-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |