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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HSC-T6 using 67021-1-Ig (same clone as 67021-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HepG2 using 67021-1-Ig (same clone as 67021-1-PBS)

    WB result of NFS1 antibody (67021-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of NIH/3T3 using 67021-1-Ig (same clone as 67021-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of RAW 264.7 using 67021-1-Ig (same clone as 67021-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HeLa using 67021-1-Ig (same clone as 67021-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HEK-293 using 67021-1-Ig (same clone as 67021-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HepG2 using 67021-1-Ig (same clone as 67021-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NFS1 Monoclonal antibody, PBS Only

NFS1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67021-1-PBS
Clone No.3A10A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

3A10A7, Cysteine desulfurase, EC:2.8.1.7, HUSSY 08, HUSSY-08

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67021-1-PBS targets NFS1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8017

Product name: Recombinant human NFS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-189 aa of BC018471

Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF

相同性解析による交差性が予測される生物種
Full Name NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC018471
Gene Symbol NFS1
Gene ID (NCBI) 9054
RRIDAB_2918480
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9Y697
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.