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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFRKB Polyclonal antibody (14086-1-AP)

    WB analysis using 14086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14086-1-AP (NFRKB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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NFRKB Polyclonal antibody

NFRKB Polyclonal Antibody for WB, ELISA
Cat No. 14086-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Nuclear factor related to kappa-B-binding protein, INO80G, INO80 complex subunit G, DNA-binding protein R kappa-B, DNA binding protein R kappa B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBGC-823 cells, HepG2 cells, Jurkat cells, C2C12 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14086-1-AP targets NFRKB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFRKB fusion protein Ag5216 相同性解析による交差性が予測される生物種
Full Name nuclear factor related to kappaB binding protein
Calculated molecular weight 1299 aa, 139 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC063280
Gene Symbol NFRKB
Gene ID (NCBI) 4798
RRIDAB_3669177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P4R8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFRKB (Nuclear Factor Related to KappaB Binding Protein) plays a role in the recombination of DNA, it helps in the regulation of transcription, DNA-templated, and transcription by RNA polymerase II, and is involved in the regulation of inflammatory responses. It also regulates protein deubiquitination, which is important for protein stability and function.

Protocols

Product Specific Protocols
WB protocol for NFRKB antibody 14086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols