Validation Data Gallery
|Positive WB detected in
|A431 cells, HeLa cells, HEK-293 cells, pig liver tissue, HL-60 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells
|Positive IP detected in
|Positive IHC detected in
|human stomach tissue, mouse kidney tissue, human prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|A431 cells, HeLa cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Sample-dependent, check data in validation data gallery
10268-1-AP targets IkB Alpha in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|human, mouse, pig, rat
|human, CHICKEN, rat, mouse, ducks, pig, bovine
|Host / Isotype
|Rabbit / IgG
|IkB Alpha fusion protein Ag0345 相同性解析による交差性が予測される生物種
|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
|Product Specific Protocols
|WB protocol for IkB Alpha antibody 10268-1-AP
|IHC protocol for IkB Alpha antibody 10268-1-AP
|IF protocol for IkB Alpha antibody 10268-1-AP
|IP protocol for IkB Alpha antibody 10268-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SONAM (Verified Customer) (10-04-2023)
Antibody worked well. No nonspecific band was observed.
CHAO (Verified Customer) (01-30-2020)
Weak bands for the heart lysates at basal state.