IkB Alpha Polyclonal antibody
IkB Alpha Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (3)
Applications
WB, IHC, IF, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
500
Cat no : 10268-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, pig liver tissue, HL-60 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human stomach tissue, mouse kidney tissue, human prostate cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 479 publications below |
IHC | See 14 publications below |
IF | See 7 publications below |
FC | See 1 publications below |
CoIP | See 2 publications below |
Product Information
10268-1-AP targets IkB Alpha in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig, chicken, bovine, ducks |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IkB Alpha fusion protein Ag0345 相同性解析による交差性が予測される生物種 |
Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 36 kDa |
GenBank accession number | BC002601 |
Gene symbol | IkB Alpha |
Gene ID (NCBI) | 4792 |
RRID | AB_2151423 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Protocols
Product Specific Protocols | |
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WB protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
IHC protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
IF protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
IP protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Nucleic Acids Res p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway | ||
Sci Adv Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SONAM (Verified Customer) (10-04-2023) | Antibody worked well. No nonspecific band was observed.
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FH CHAO (Verified Customer) (01-30-2020) | Weak bands for the heart lysates at basal state.
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