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NFKB2 Polyclonal antibody

NFKB2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10409-2-AP

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NF kappa B, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100



Tested Applications

Positive WB detected inmulti-cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10409-2-AP targets NFKB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFKB2 fusion protein Ag0662 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Calculated molecular weight 97 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC002844
Gene symbol NFKB2
Gene ID (NCBI) 4791
RRIDAB_2151261
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody recognizes both p52 and p100.

Protocols

Product Specific Protocols
WB protocol for NFKB2 antibody 10409-2-APDownload protocol
IHC protocol for NFKB2 antibody 10409-2-APDownload protocol
IF protocol for NFKB2 antibody 10409-2-APDownload protocol
IP protocol for NFKB2 antibody 10409-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

A proteomics landscape of circadian clock in mouse liver.

Authors - Yunzhi Wang
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
mouseWB

J Hazard Mater

TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.

Authors - Shuang Li
humanWB

PLoS Pathog

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Authors - Muyu Xu
humanWB

Cell Death Dis

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Authors - Xinhua Xiao
humanWB

Mol Ther Nucleic Acids

circPUM1 Promotes Tumorigenesis and Progression of Ovarian Cancer by Sponging miR-615-5p and miR-6753-5p.

Authors - Xue Guan