Validation Data Gallery
|Positive WB detected in
|multi-cells, HeLa cells
|Positive IP detected in
|Positive IHC detected in
|human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
|IHC : 1:20-1:200
|IF : 1:10-1:100
|Sample-dependent, check data in validation data gallery
10409-2-AP targets NFKB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, rat, mouse
|Host / Isotype
|Rabbit / IgG
|NFKB2 fusion protein Ag0662 相同性解析による交差性が予測される生物種
|nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody recognizes both p52 and p100.
|Product Specific Protocols
|WB protocol for NFKB2 antibody 10409-2-AP
|IHC protocol for NFKB2 antibody 10409-2-AP
|IF protocol for NFKB2 antibody 10409-2-AP
|IP protocol for NFKB2 antibody 10409-2-AP
|Click here to view our Standard Protocols
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