Validation Data Gallery
|Positive WB detected in
|A431 cells, HeLa cells, Jurkat cells, Raji cells, U-87 MG cells
|Positive IP detected in
|Positive IF detected in
|HeLa cells, HepG2 cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
|IF : 1:200-1:800
|Sample-dependent, check data in validation data gallery
14220-1-AP targets NFKB1,p105,p50 in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.
|human, CHICKEN, rat, mouse, ducks, monkey, pig, canine, bovine
|Host / Isotype
|Rabbit / IgG
|NFKB1,p105,p50 fusion protein Ag5458 相同性解析による交差性が予測される生物種
|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
|Calculated molecular weight
|Observed molecular weight
|50 kDa, 105 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.
|Product Specific Protocols
|WB protocol for NFKB1,p105,p50 antibody 14220-1-AP
|IF protocol for NFKB1,p105,p50 antibody 14220-1-AP
|IP protocol for NFKB1,p105,p50 antibody 14220-1-AP
|FC protocol for NFKB1,p105,p50 antibody 14220-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ane (Verified Customer) (09-18-2023)
This antibody was used to detect p50 at different cell compartments and worked fine to dectect both phosphorilated and unphosphorilated forms of p50 in all cell compartments