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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFE2 Monoclonal antibody

NFE2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D7C8

Cat no : 66436-1-Ig

Synonyms

Leucine zipper protein NF E2, NF E2, NFE2, p45, p45 NF E2

Validation Data Gallery

Applications:
  • WB analysis of HL-60 using 66436-1-Ig

    WB analysis of HL-60 using 66436-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 66436-1-Ig

    WB analysis of K-562 using 66436-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHL-60 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy NFE2 Ready-To-Use IHC Kit. NFE2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

66436-1-Ig targets NFE2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFE2 fusion protein Ag1570 相同性解析による交差性が予測される生物種
Full Name nuclear factor (erythroid-derived 2), 45kDa
Calculated molecular weight 41 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC005044
Gene symbol NFE2
Gene ID (NCBI) 4778
RRIDAB_2881806
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Protocols

Product Specific Protocols
WB protocol for NFE2 antibody 66436-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols