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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFATC4 Polyclonal antibody (26311-1-AP)

    WB analysis using 26311-1-AP (same clone as 26311-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26311-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NFATC4 Polyclonal antibody (26311-1-AP)

    WB analysis using 26311-1-AP (same clone as 26311-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26311-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NFATC4 Polyclonal antibody (26311-1-AP)

    IHC staining of human lung cancer using 26311-1-AP (same clone as 26311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26311-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NFATC4 Polyclonal antibody (26311-1-AP)

    IHC staining of human lung cancer using 26311-1-AP (same clone as 26311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26311-1-PBS in a different storage buffer formulation.

NFATC4 Polyclonal antibody, PBS Only

NFATC4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 26311-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26311-1-PBS targets NFATC4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24503

Product name: Recombinant human NFATC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-225 aa of BC053855

Sequence: GAGGAGGGRVLECPSIRITSISPTPEPPAALEDNPDAWGDGSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFGLGSPLPSPRASPRPW

相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
Calculated molecular weight 95 kDa
Observed molecular weight 90-105 kDa
GenBank accession numberBC053855
Gene Symbol NFATC4
Gene ID (NCBI) 4776
RRIDAB_3085854
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14934
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NFATC4, also named NFAT3, is a member from the Nuclear Factor of Activated T cells (NFATs) transcription factor family. NFATc4 contains a conserved Rel homology domain (RHD) which includes the DNA-binding motif, and two transactivation domains (TADs) which locate at the N-terminus and C-terminus respectively. NFATC4 is involved in several physiological processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. It is also reported that NFATC4 could take part in cell growth, cell migration, tumor metastasis, and patient survival recently.

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