CoraLite®594-conjugated NFATC1 Monoclonal antibody
NFATC1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E1B10
Cat no : CL594-66963
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-66963 targets NFATC1 in FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | NFATC1 fusion protein Ag13014 相同性解析による交差性が予測される生物種 |
Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 |
Calculated molecular weight | 716aa,78 kDa; 943aa,101 kDa |
Observed molecular weight | 101 kDa |
GenBank accession number | BC104753 |
Gene symbol | NFATC1 |
Gene ID (NCBI) | 4772 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).
Protocols
Product Specific Protocols | |
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FC protocol for CL594 NFATC1 antibody CL594-66963 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |