Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 21713-1-AP (same clone as 21713-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of human urothelial carcinoma using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of rat skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21713-1-AP (same clone as 21713-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFAT5 antibody (21713-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

NFAT5 Polyclonal antibody, PBS Only

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21713-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21713-1-PBS targets NFAT5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16341 Product name: Recombinant human NFAT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1231-1531 aa of BC146765 Sequence: MFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF 相同性解析による交差性が予測される生物種
Full Name	nuclear factor of activated T-cells 5, tonicity-responsive
Calculated molecular weight	1531 aa, 166 kDa
Observed molecular weight	200 kDa
GenBank accession number	BC146765
Gene Symbol	NFAT5
Gene ID (NCBI)	10725
RRID	AB_3085667
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94916
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of human urothelial carcinoma using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of rat skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)

IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)