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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of HEK-293 using 66642-1-Ig (same clone as 66642-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of Y79 using 66642-1-Ig (same clone as 66642-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of Neuro-2a using 66642-1-Ig (same clone as 66642-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of HeLa using 66642-1-Ig (same clone as 66642-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of mouse brain using 66642-1-Ig (same clone as 66642-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NEUROG1 Monoclonal antibody (66642-1-Ig)

    IF Staining of rat brain using 66642-1-Ig (same clone as 66642-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NEUROG1 antibody (66642-1-Ig, Clone: 1E1H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66642-1-PBS in a different storage buffer formulation.

NEUROG1 Monoclonal antibody, PBS Only

NEUROG1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 66642-1-PBS
Clone No.1E1H6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

1E1H6, AKA, bHLHa6, Class A basic helix-loop-helix protein 6, Math4C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66642-1-PBS targets NEUROG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26438

Product name: Recombinant human NEUROG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-237 aa of BC008687

Sequence: MPARLETCISDLDCASSSGSDLSGFLTDEEDCARLQQAASASGPPAPARRGAPNISRASEVPGAQDDEQERRRRRGRTRVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNYIWALAETLRLADQGLPGGGARERLLPPQCVPCLPGPPSPASDAESWGSGAAAASPLSDPSSPAASEDFTYRPGDPVFSFPSLPKDLLHTTPCFIPYH

相同性解析による交差性が予測される生物種
Full Name neurogenin 1
Calculated molecular weight 237 aa, 26 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008687
Gene Symbol NEUROG1
Gene ID (NCBI) 4762
RRIDAB_2882001
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ92886
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NEUROG1, also named BHLHA6, NEUROD3, NGN, and NGN1, is a 237 amino acid protein, which is expressed in embryonic nervous system. NEUROG1 acts as a transcriptional regulator that is involved in the initiation of neuronal differentiation. It activates transcription by binding to the E box (5'-CANNTG-3'). NEUROG1 can associate with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

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