Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
14610-1-AP targets NEUROD4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6183 Product name: Recombinant human NEUROD4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-255 aa of BC040961 Sequence: MTKARLERFRARRVKANARERTRMHGLNDALDNLRRVMPCYSKTQKLSKIETLRLARNYIWALSEVLETGQTPEGKGFVEMLCKGLSQPTSNLVAGCLQLGPQSVLLEKHEDKSPICDSAISVHNFNYQSPGLPSPPYGHMETHLLHLKPQVFKSLGESSFGSHLPDCSTPPYEGPLTPPLSISGNFSLKQDGSPDLEKSYSFMPHYPSSSLSSGHVHSTPFQAGTPRYDVPIDMSYDSYPHHGIGTQLNTVFTE 相同性解析による交差性が予測される生物種 |
| Full Name | neurogenic differentiation 4 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC040961 |
| Gene Symbol | NEUROD4 |
| Gene ID (NCBI) | 58158 |
| RRID | AB_2878069 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NEUROD4 is a basic helix-loop-helix (bHLH) transcription factor encoded by the NEUROD4 gene on human chromosome 12q13.2. It mediates neuronal differentiation and is required for amacrine cell fate specification in the retina. Expression is enriched in brain, adrenal gland, and retina. Orthologs exist in mouse (Math3), rat, zebrafish, and chick.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEUROD4 antibody 14610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Spinal cord Tau pathology induces tactile deficits and cognitive impairment in Alzheimer's disease via dysregulation of CCK neurons | ||
Nat Commun VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination. |