Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, NCI-H1299 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55345-1-AP targets NEURL4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | neuralized homolog 4 (Drosophila) |
| Calculated molecular weight | 167 kDa |
| Observed molecular weight | 166 kDa |
| GenBank accession number | NM_032442 |
| Gene Symbol | NEURL4 |
| Gene ID (NCBI) | 84461 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NEURL4, also known as KIAA1787, has 6 highly conserved neuralized homology repeats (NHRs), which are thought to mediate protein-protein interactions. NEURL4 localizes to the daughter centriole and prevents formation of ectopic microtubule-organizing centers. NEURL4 promotes CCP110 ubiquitination and proteasome-dependent degradation, maintaining normal centrosomal homeostasis and preventing the formation of abnormal microtubule tissue centers (MTOCs) by countering CP110 accumulation(PMID: 22441691, 22261722).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEURL4 antibody 55345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |