Validation Data Gallery
Tested Applications
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29936-1-AP targets NETO1 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32177 Product name: Recombinant human NETO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-213 aa of NM_138999 Sequence: TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSK 相同性解析による交差性が予測される生物種 |
| Full Name | neuropilin (NRP) and tolloid (TLL)-like 1 |
| Calculated molecular weight | 60 kDa |
| GenBank accession number | NM_138999 |
| Gene Symbol | NETO1 |
| Gene ID (NCBI) | 81832 |
| RRID | AB_3742322 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The NETO1 protein (neuropilin and tolloid-like protein 1) is a member of the NETO family. It is a type I transmembrane protein primarily expressed in the central nervous system. Serving as an important auxiliary subunit of glutamate receptors (especially kainate receptors and NMDA receptors), NETO1 plays a critical role in synaptic plasticity, learning, and memory by regulating receptor trafficking, gating properties, and synaptic localization. Its abnormalities are associated with neuropsychiatric disorders such as schizophrenia and epilepsy.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NETO1 antibody 29936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |