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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using NET1 Polyclonal antibody (12740-1-AP)

    WB analysis of Y79 using 12740-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12740-1-AP (NET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of mouse heart using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of mouse stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of rat heart using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of rat stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using NET1 Polyclonal antibody (12740-1-AP)

    IHC staining of rat stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NET1 Polyclonal antibody (12740-1-AP)

    IF Staining of A431 using 12740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NET1 antibody (12740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NET1 Polyclonal antibody

NET1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12740-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ARHGEF8, NET1A, Neuroepithelial cell-transforming gene 1 protein, Proto oncogene p65 Net1, Proto-oncogene p65 Net1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inhuman stomach tissue, mouse stomach tissue, mouse heart tissue, rat heart tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12740-1-AP targets NET1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3636

Product name: Recombinant human NET1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 142-543 aa of BC010285

Sequence: ARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV

相同性解析による交差性が予測される生物種
Full Name neuroepithelial cell transforming 1
Calculated molecular weight 596 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC010285
Gene Symbol NET1
Gene ID (NCBI) 10276
RRIDAB_2923558
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for NET1 antibody 12740-1-APDownload protocol
IHC protocol for NET1 antibody 12740-1-APDownload protocol
WB protocol for NET1 antibody 12740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Oncol Rep

miR‑22‑3p enhances multi‑chemoresistance by targeting NET1 in bladder cancer cells.

Authors - Jun Xiao
View Article
humanIHC

Hum Pathol

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation.

Authors - Yuting Zhang
View Article
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