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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NET1 Polyclonal antibody

NET1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12740-1-AP

Synonyms

ARHGEF8, NET1, NET1A, Proto oncogene p65 Net1

Validation Data Gallery

Applications:
  • WB analysis of Y79 using 12740-1-AP

    WB analysis of Y79 using 12740-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12740-1-AP (NET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 12740-1-AP

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 12740-1-AP

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of A431 using 12740-1-AP

    IF Staining of A431 using 12740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NET1 antibody (12740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

12740-1-AP targets NET1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NET1 fusion protein Ag3636 相同性解析による交差性が予測される生物種
Full Name neuroepithelial cell transforming 1
Calculated molecular weight 596 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC010285
Gene symbol NET1
Gene ID (NCBI) 10276
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NET1 antibody 12740-1-APDownload protocol
IHC protocol for NET1 antibody 12740-1-APDownload protocol
IF protocol for NET1 antibody 12740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Pathol

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation.

Authors - Yuting Zhang
View Article