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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (30760-1-AP)

    WB analysis using 30760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30760-1-AP (Nestin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Nestin Polyclonal antibody (30760-1-AP)

    IHC staining of rat cerebellum using 30760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30760-1-AP (Nestin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Nestin Polyclonal antibody (30760-1-AP)

    IF Staining of mouse brain using 30760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NES antibody (30760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Nestin Polyclonal antibody (30760-1-AP)

    IF Staining of SH-SY5Y using 30760-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Nestin antibody (30760-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Nestin Polyclonal antibody

Nestin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 30760-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

NES

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSGC-7901 cells, SH-SY5Y cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30760-1-AP targets Nestin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29600

Product name: Recombinant human NES protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-276 aa of NM_006617

Sequence: MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDELAALRALVDQRWREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEKCARAWLSSQVAELERELEALRVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQARERLGRAVQGAREGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQS

相同性解析による交差性が予測される生物種
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight240-280 kDa
GenBank accession numberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_3086411
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Protocols

Product Specific Protocols
IF protocol for Nestin antibody 30760-1-APDownload protocol
IHC protocol for Nestin antibody 30760-1-APDownload protocol
WB protocol for Nestin antibody 30760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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