Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells, Ramos cells, SH-SY5Y cells, SK-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33450-1-AP targets NEPRO in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38276 Product name: Recombinant human C3orf17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-168 aa of NM_015412.3 Sequence: MMAAVPPGLEPWNRVRIPKAGNRSAVTVQNPGAALDLCIAAVIKECHLVILSLKSQTLDAETDVLCAVLYSNHNRMGRHKPHLALKQVEQCLKRLKNMNLEGSIQDLFELFSSNENQPLTTKVCVVPSQPVVELVLMKVLGACKLLLRLLDCCCKTFLLTVKHLGLQE 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 3 open reading frame 17 |
| Calculated molecular weight | 65kDa，567aa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | NM_015412.3 |
| Gene Symbol | C3orf17 |
| Gene ID (NCBI) | 25871 |
| RRID | AB_3742976 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6NW34 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The NEPRO protein (full name: Nucleolus and Neural Progenitor Protein) belongs to the NEPRO family and is primarily localized in the nucleolus. Its core function is to serve as a key downstream factor of the Notch signaling pathway during cerebral cortex development, where it maintains the stemness of neural progenitor cells by inhibiting their differentiation into neurons, thereby ensuring normal development of the nervous system. Additionally, this protein also plays an indispensable role during pre-implantation embryonic development in mice (PMID: 26178919).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEPRO antibody 33450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |