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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NELL2 Polyclonal antibody (11268-1-AP)

    WB analysis using 11268-1-AP (same clone as 11268-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11268-1-AP (NELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11268-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NELL2 Polyclonal antibody (11268-1-AP)

    IHC staining of mouse brain using 11268-1-AP (same clone as 11268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11268-1-AP (NELL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11268-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NELL2 Polyclonal antibody (11268-1-AP)

    IHC staining of mouse brain using 11268-1-AP (same clone as 11268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11268-1-AP (NELL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11268-1-PBS in a different storage buffer formulation.

NELL2 Polyclonal antibody, PBS Only

NELL2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11268-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

NEL like protein 2, Nel related protein 2, NEL-like protein 2, Nel-related protein 2, NRP2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11268-1-PBS targets NELL2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1793

Product name: Recombinant human NELL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 443-740 aa of BC020544

Sequence: ECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVE

相同性解析による交差性が予測される生物種
Full Name NEL-like 2 (chicken)
Calculated molecular weight 91 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC020544
Gene Symbol NELL2
Gene ID (NCBI) 4753
RRIDAB_10638004
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99435
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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