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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEK7 Polyclonal antibody (31422-1-AP)

    WB analysis using 31422-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NEK7 Polyclonal antibody (31422-1-AP)

    WB analysis using 31422-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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NEK7 Polyclonal antibody

NEK7 Polyclonal Antibody for WB, ELISA
Cat No. 31422-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NimA related protein kinase 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC6 cells, HeLa cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31422-1-AP targets NEK7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK7 fusion protein Ag33629 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)-related kinase 7
Calculated molecular weight35
Observed molecular weight 32-35 kDa
GenBank accession numberNM_133494
Gene Symbol NEK7
Gene ID (NCBI) 140609
RRIDAB_3669975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8TDX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian NIMA-related kinases (NEKs) represent a family of serine/threonine kinases, named NEK1-NEK11, which are implicated in the control of several aspects of mitosis and are involved in non-mitotic functions (PMID: 33364979). NIMA-related kinase 7 (NEK7) is the smallest NIMA-related kinase (NEK) in mammals. NEK7 is identified as a highly conserved serine or threonine kinase, which is not only crucial for mitosis entry, cell cycle progression, cell division, and mitotic process, but also expressed in brain, heart, lung, liver, spleen, and other issues (PMID: 31787755).

Protocols

Product Specific Protocols
WB protocol for NEK7 antibody 31422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols