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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEK5 Polyclonal antibody

NEK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26254-1-AP

Synonyms

NEK5, NimA related protein kinase 5

Validation Data Gallery

Applications:
  • WB analysis using 26254-1-AP

    WB analysis using 26254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26254-1-AP (NEK5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26254-1-AP

    IHC staining of human thyroid cancer using 26254-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26254-1-AP (NEK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMDA-MB-231 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

26254-1-AP targets NEK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK5 fusion protein Ag24419 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)-related kinase 5
Calculated molecular weight 708 aa, 81 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC063885
Gene symbol NEK5
Gene ID (NCBI) 341676
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NEK5 antibody 26254-1-APDownload protocol
IHC protocol for NEK5 antibody 26254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols