• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NEK1 Polyclonal antibody

NEK1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27146-1-AP

Synonyms

KIAA1901, NEK1, NimA related protein kinase 1, NY REN 55

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 27146-1-AP

    WB analysis of HeLa using 27146-1-AP

    WB result of NEK1 antibody (27146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK1 transfected HeLa cells.

  • WB analysis of PC-12 using 27146-1-AP

    WB analysis of PC-12 using 27146-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 27146-1-AP

    WB analysis of mouse pancreas using 27146-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 27146-1-AP

    IP experiment of HeLa using 27146-1-AP

    HeLa lysates prepared and IP of NEK1 performed using 1.0 μg of 27146-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IP experiment of PC-12 using 27146-1-AP

    IP experiment of PC-12 using 27146-1-AP

    IP result of anti-NEK1 (IP:27146-1-AP, 4ug; Detection:27146-1-AP 1:500) with PC-12 cells lysate 3080 ug.

  • IHC staining of human lung cancer using 27146-1-AP

    IHC staining of human lung cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27146-1-AP

    IHC staining of human lung cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27146-1-AP

    IF Staining of HeLa using 27146-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NEK1 antibody (27146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-12 cells, mouse pancreas tissue, HeLa cells
Positive IP detected inPC-12 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

27146-1-AP targets NEK1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK1 fusion protein Ag25970 相同性解析による交差性が予測される生物種
Full Name NIMA (never in mitosis gene a)-related kinase 1
Calculated molecular weight 1258 aa, 143 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC114491
Gene symbol NEK1
Gene ID (NCBI) 4750
RRIDAB_2880773
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NEK1 antibody 27146-1-APDownload protocol
IHC protocol for NEK1 antibody 27146-1-APDownload protocol
IF protocol for NEK1 antibody 27146-1-APDownload protocol
IP protocol for NEK1 antibody 27146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article
humanWB,IHC

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen
View Article