Validation Data Gallery

Filter: AllWB

WB analysis using 11621-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11621-1-AP
WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells.
WB analysis of HEK-293 using 11621-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skin using 11621-1-AP
human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11621-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11621-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 11621-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

NEIL3 Polyclonal antibody

NEIL3 Polyclonal Antibody for WB, ELISA

Cat No. 11621-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP, ELISA

Synonyms

DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, EC:3.2.2.-, Endonuclease 8 like 3, Endonuclease 8-like 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

HEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 13 publications below
IP	See 3 publications below
ChIP	See 1 publications below

Product Information

11621-1-AP targets NEIL3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2200 Product name: Recombinant human NEIL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 257-605 aa of BC025954 Sequence: CHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC 相同性解析による交差性が予測される生物種
Full Name	nei endonuclease VIII-like 3 (E. coli)
Calculated molecular weight	605 aa, 68 kDa
Observed molecular weight	62-68 kDa
GenBank accession number	BC025954
Gene Symbol	NEIL3
Gene ID (NCBI)	55247
RRID	AB_2251229
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TAT5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

Protocols

Product Specific Protocols
WB protocol for NEIL3 antibody 11621-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. Authors - Dan Liu View Article
human	WB	Nucleic Acids Res Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair. Authors - Niu Li KD Validated View Article
human	WB	EMBO J PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. Authors - Daniel González-Acosta View Article
human	WB	Sci Rep NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells Authors - Umit Aliyaskarova View Article
human	WB,IP	DNA Repair (Amst) Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway. Authors - Neurauter Christine Gran CG View Article
human	WB,IP,ChIP	Cell Rep NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis. Authors - Jia Zhou KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11621-1-AP

WB analysis of HEK-293 using 11621-1-AP

WB analysis of HEK-293 using 11621-1-AP

WB analysis of human skin using 11621-1-AP

WB analysis of HEK-293 using 11621-1-AP

WB analysis of A431 using 11621-1-AP

WB analysis of mouse skin using 11621-1-AP

NEIL3 Polyclonal antibody

NEIL3 Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.

PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.

NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.