• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NEDD4L Polyclonal antibody (13690-1-AP)

    WB analysis using 13690-1-AP (same clone as 13690-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using NEDD4L Polyclonal antibody (13690-1-AP)

    WB analysis of C6 using 13690-1-AP (same clone as 13690-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using NEDD4L Polyclonal antibody (13690-1-AP)

    WB analysis of HT-1080 using 13690-1-AP (same clone as 13690-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NEDD4L Polyclonal antibody (13690-1-AP)

    WB analysis of PC-3 using 13690-1-AP (same clone as 13690-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of C6 cells using NEDD4L Polyclonal antibody (13690-1-AP)

    IP experiment of C6 using 13690-1-AP (same clone as 13690-1-PBS)

    IP result of anti-NEDD4L (IP:13690-1-AP, 4ug; Detection:13690-1-AP 1:300) with C6 cells lysate 1000 ug. This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human stomach cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13690-1-AP (NEDD4L antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human prostate cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human prostate cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human stomach cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human stomach cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human prostate cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NEDD4L Polyclonal antibody (13690-1-AP)

    IHC staining of human prostate cancer using 13690-1-AP (same clone as 13690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using NEDD4L Polyclonal antibody (13690-1-AP)

    IF Staining of PC-3 using 13690-1-AP (same clone as 13690-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (13690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13690-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NEDD4L Polyclonal antibody, PBS Only

NEDD4L Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13690-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase NEDD4-like, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NED4L, hNEDD4-2, KIAA0439

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13690-1-PBS targets NEDD4L in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4565

Product name: Recombinant human NEDD4L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 565-911 aa of BC032597

Sequence: EESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Calculated molecular weight 854 aa, 96 kDa
Observed molecular weight 112 kDa, 125 kDa
GenBank accession numberBC032597
Gene Symbol NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2149326
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PU5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

||
New chat

Able™

正在加载，请稍候...