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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD1 Recombinant antibody (83443-6-RR)

    WB analysis using 83443-6-RR (same clone as 83443-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NEDD1 Recombinant antibody (83443-6-RR)

    IF Staining of HeLa using 83443-6-RR (same clone as 83443-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEDD1 antibody (83443-6-RR, Clone: 240404A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using NEDD1 Recombinant antibody (83443-6-RR)

    FC experiment of HEK-293 using 83443-6-RR (same clone as 83443-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NEDD1 Recombinant antibody (83443-6-RR)

    FC experiment of A431 using 83443-6-RR (same clone as 83443-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83443-6-RR

    Affinity and Kinetic Characterization of 83443-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

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NEDD1 Recombinant antibody, PBS Only

NEDD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83443-6-PBS
Clone No.240404A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240404A7, NEDD 1, NEDD-1, Neural precursor cell expressed developmentally down-regulated protein 1, Protein NEDD1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83443-6-PBS targets NEDD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NEDD1 fusion protein Ag33603 相同性解析による交差性が予測される生物種
Full Name neural precursor cell expressed, developmentally down-regulated 1
Calculated molecular weight 72 kDa
Observed molecular weight71-75 kDa
GenBank accession numberBC027605
Gene Symbol NEDD1
Gene ID (NCBI) 121441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NHV4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NEDD1 (NEDD1 gamma-tubulin ring complex targeting factor), also known as GCP-WD. It is expected to be located in centrosome; nucleoplasm; and plasma membrane. Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. The molecular weight of NEDD1is 72 kDa.