Validation Data Gallery

Filter: AllWB

WB analysis of MCF-7 using 15899-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15899-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 15899-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15899-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15899-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15899-1-AP (NECAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NECAP2 Polyclonal antibody

NECAP2 Polyclonal Antibody for WB, ELISA

Cat No. 15899-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

NECAP 2, NECAP endocytosis associated 2, NECAP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

MCF7 cells, HeLa cells, human liver tissue, mouse cerebellum tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

15899-1-AP targets NECAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8721 Product name: Recombinant human NECAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC018914 Sequence: MEESGYESVLCVKPDVHVYRIPPRATNRGYRAAEWQLDQPSWSGRLRITAKGQMAYIKLEDRTSGELFAQAPVDQFPGTAVESVTDSSRYFVIRIEDGNGRRAFIGIGFGDRGDAFDFNVALQDHFKWVKQQCEFAKQAQNPDQGPKLDLGFKEGQTIKLNIANMKKKEGAAGNPRVRPASTGGLSLLPPPPGGKTSTLIPPPGEQLAVGGSLVQPAVAPSSGGAPVPWPQPNPATADIWGDFTKSTGSTSSQTQPGTGWVQF 相同性解析による交差性が予測される生物種
Full Name	NECAP endocytosis associated 2
Calculated molecular weight	263 aa, 28 kDa
Observed molecular weight	35-37 kDa
GenBank accession number	BC018914
Gene Symbol	NECAP2
Gene ID (NCBI)	55707
RRID	AB_2149187
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NVZ3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NECAP2 antibody 15899-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Oxid Med Cell Longev Comprehensive Prognostic Analysis of Immune Implication Value and Oxidative Stress Significance of NECAP2 in Low-Grade Glioma Authors - Zhichao Lu View Article

製品

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カスタム対応

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企業情報

抗体

免疫アッセイ

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ChromoTek VHH（Nanobody®）試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate