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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    WB analysis of human brain using 18940-1-AP (same clone as 18940-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18940-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    WB analysis of human brain using 18940-1-AP (same clone as 18940-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18940-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    WB analysis of rat brain using 18940-1-AP (same clone as 18940-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    IHC staining of human pancreas using 18940-1-AP (same clone as 18940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18940-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NECAB3 Polyclonal antibody (18940-1-AP)

    IHC staining of human pancreas using 18940-1-AP (same clone as 18940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18940-1-PBS in a different storage buffer formulation.

NECAB3 Polyclonal antibody, PBS Only

NECAB3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18940-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18940-1-PBS targets NECAB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13493

Product name: Recombinant human NECAB3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-362 aa of BC047673

Sequence: MACAGLLTVCLLRPPAPQPQPQTPRHPQLAPDPGPAGHTLFQDVFRRADKNDDGKLSFEEFQNYFADGVLSLGELQELFSGIDGHLTDNLETEKLCDYFSEHLGVYRPVLAALESLNRAVLAAMDATKLEYERASKVDQFVTRFLLRETVSQLQALQSSLEGASDTLEAQAHGWRSDAESVEAQSRLCGSRRAGRRALRSVSRSSTWSPGSSDTGRSSEAEMQWRLQVNRLQELIDQLECKAPRLEPLREEDLAKGPDLHILMAQRQVQVAEEGLQDFHRALRCYVDFTGAQSHCLHVSAQKMLDGASFTLYEFWQDEASWRRHQQSPGSKAFQRILIDHLRAPDTLTTVFFPASWWIMNNN

相同性解析による交差性が予測される生物種
Full Name N-terminal EF-hand calcium binding protein 3
Calculated molecular weight362aa,41 kDa; 396aa,44 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC047673
Gene Symbol NECAB3
Gene ID (NCBI) 63941
RRIDAB_10596622
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P71
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NECAB3, also named as APBA2BP, NIP1, SYTIP2 and XB51, is a potential substrate of Nek2. NECAB3 inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP). NECAB3 has three isoforms with MW 40 kDa, 44 kDa and 22 kDa.

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