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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFV1 Monoclonal antibody (68144-1-Ig)

    WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NDUFV1 Monoclonal antibody (68144-1-Ig)

    WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NDUFV1 Monoclonal antibody (68144-1-Ig)

    WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using NDUFV1 Monoclonal antibody (68144-1-Ig)

    WB analysis of 4T1 using 68144-1-Ig (same clone as 68144-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NDUFV1 Monoclonal antibody (68144-1-Ig)

    IHC staining of human lung cancer using 68144-1-Ig (same clone as 68144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NDUFV1 Monoclonal antibody (68144-1-Ig)

    IHC staining of human lung cancer using 68144-1-Ig (same clone as 68144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NDUFV1 Monoclonal antibody (68144-1-Ig)

    IHC staining of mouse skeletal muscle using 68144-1-Ig (same clone as 68144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NDUFV1 Monoclonal antibody (68144-1-Ig)

    IHC staining of mouse skeletal muscle using 68144-1-Ig (same clone as 68144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

NDUFV1 Monoclonal antibody, PBS Only

NDUFV1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68144-1-PBS
Clone No.3E9D4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68144-1-PBS targets NDUFV1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

Tested Reactivity Human, pig, rabbit, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag32659

Product name: Recombinant human NDUFV1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 116-447 aa of BC015645

Sequence: NADEGEPGTCKDREILRHDPHKLLEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGTWFAGFGRERNSGTKLFNISGHVNHPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALVQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVRGDARPAEIDSLWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELE

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Calculated molecular weight 51 kDa
Observed molecular weight45 kDa
GenBank accession numberBC015645
Gene Symbol NDUFV1
Gene ID (NCBI) 4723
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP49821
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

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