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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using NDUFS7 Polyclonal antibody (15728-1-AP)

    WB analysis of A549 using 15728-1-AP (same clone as 15728-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using NDUFS7 Polyclonal antibody (15728-1-AP)

    WB analysis of human brain using 15728-1-AP (same clone as 15728-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFS7 Polyclonal antibody (15728-1-AP)

    IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFS7 Polyclonal antibody (15728-1-AP)

    IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFS7 Polyclonal antibody (15728-1-AP)

    IF Staining of HeLa using 15728-1-AP (same clone as 15728-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS7 antibody (15728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

NDUFS7 Polyclonal antibody, PBS Only

NDUFS7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15728-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15728-1-PBS targets NDUFS7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8471

Product name: Recombinant human NDUFS7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC005954

Sequence: MAVLSAPGLRGFRILGLRSSVGLAVQARGVHQSVATDGPSSTQPALPKARAVAPKPSSRGEYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYIPGCPPTAEALLYGILQLQRKIKRERRLQIWYRR

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 213 aa, 24 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC005954
Gene Symbol NDUFS7
Gene ID (NCBI) 374291
RRIDAB_2149029
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75251
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFS7(NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial) protein is one of the most conserved subunits of mitochondrial respiratory chain complex I and plays a central role in the interaction with the electron acceptor ubiquinone and in the proton-translocating mechanism(PMID:15269216).Defects in NDUFS7 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

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