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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS6 Polyclonal antibody (14417-1-AP)

    WB analysis using 14417-1-AP (same clone as 14417-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NDUFS6 Polyclonal antibody (14417-1-AP)

    WB analysis using 14417-1-AP (same clone as 14417-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using NDUFS6 Polyclonal antibody (14417-1-AP)

    WB analysis of rat liver using 14417-1-AP (same clone as 14417-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using NDUFS6 Polyclonal antibody (14417-1-AP)

    WB analysis of human heart using 14417-1-AP (same clone as 14417-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using NDUFS6 Polyclonal antibody (14417-1-AP)

    WB analysis of human liver using 14417-1-AP (same clone as 14417-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using NDUFS6 Polyclonal antibody (14417-1-AP)

    IP experiment of mouse liver using 14417-1-AP (same clone as 14417-1-PBS)

    IP result of anti-NDUFS6 (IP:14417-1-AP, 4ug; Detection:14417-1-AP 1:300) with mouse liver tissue lysate 3200 ug. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDUFS6 Polyclonal antibody (14417-1-AP)

    IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDUFS6 Polyclonal antibody (14417-1-AP)

    IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFS6 Polyclonal antibody (14417-1-AP)

    IF Staining of HepG2 using 14417-1-AP (same clone as 14417-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFS6 Polyclonal antibody (14417-1-AP)

    IF Staining of HeLa using 14417-1-AP (same clone as 14417-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

NDUFS6 Polyclonal antibody, PBS Only

NDUFS6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14417-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14417-1-PBS targets NDUFS6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5762

Product name: Recombinant human NDUFS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-124 aa of BC046155

Sequence: MAAAMTFCRLLNRCGEAARSLPLGARCFGVRVSPTGEKVTHTGQVYDDKDYRRIRFVGRQKEVNENFAIDLIAEQPVSEVETRVIACDGGGGALGHPKVYINLDKETKTGTCGYCGLQFRQHHH

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 14 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC046155
Gene Symbol NDUFS6
Gene ID (NCBI) 4726
RRIDAB_2149024
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75380
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as 1CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

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