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NDUFS5 Monoclonal antibody

NDUFS5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66053-1-Ig
Clone No.2C2A5

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 2400 ug/ml
20 μL, 2400 ug/ml
150 μL, 2400 ug/ml

¥67,000/ 150 μL

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約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HEK-293 cells, fetal human brain tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66053-1-Ig targets NDUFS5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NDUFS5 fusion protein Ag7663 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 13 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001884
Gene Symbol NDUFS5
Gene ID (NCBI) 4725
RRIDAB_11043342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach’s NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.

Protocols

Product Specific Protocols
WB protocol for NDUFS5 antibody 66053-1-IgDownload protocol
IHC protocol for NDUFS5 antibody 66053-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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