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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® NDUFS3 Multi-Recombinant antibody (RMX00096)

    WB analysis using RMX00096

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00096 (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Multi-rAb® NDUFS3 Multi-Recombinant antibody (RMX00096)

    WB analysis using RMX00096

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00096 (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Multi-rAb® NDUFS3 Multi-Recombinant antibody (RMX00096)

    IHC staining of human placenta using RMX00096

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00096 (NDUFS3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Multi-rAb® NDUFS3 Multi-Recombinant antibody (RMX00096)

    IHC staining of human placenta using RMX00096

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00096 (NDUFS3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Multi-rAb® NDUFS3 Multi-Recombinant antibody (RMX00096)

    IF Staining of HepG2 using RMX00096

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS3 antibody (RMX00096) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), (CoraLite®594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Multi-rAb® NDUFS3 Multi-Recombinant antibody (RMX00096)

    IF Staining of HepG2 using RMX00096

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS3 antibody (RMX00096) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Multi-rAb® NDUFS3 Multi-Recombinant antibody

NDUFS3 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00096

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CI 30kD, CI30kD, CI-30kD, Complex I-30kD, EC:7.1.1.2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells, K-562 cells, mouse brain tisuue, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00096 targets NDUFS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000617
Gene Symbol NDUFS3
Gene ID (NCBI) 4722
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDO75489
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

Protocols

Product Specific Protocols
IF protocol for NDUFS3 antibody RMX00096Download protocol
IHC protocol for NDUFS3 antibody RMX00096Download protocol
WB protocol for NDUFS3 antibody RMX00096Download protocol
Standard Protocols
Click here to view our Standard Protocols
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