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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS2 Recombinant antibody (84880-1-RR)

    WB analysis using 84880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84880-1-RR (NDUFS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NDUFS2 Recombinant antibody (84880-1-RR)

    IHC staining of human stomach cancer using 84880-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NDUFS2 Recombinant antibody (84880-1-RR)

    IHC staining of human stomach cancer using 84880-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NDUFS2 Recombinant antibody (84880-1-RR)

    IHC staining of mouse liver using 84880-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NDUFS2 Recombinant antibody (84880-1-RR)

    IHC staining of mouse liver using 84880-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using NDUFS2 Recombinant antibody (84880-1-RR)

    FC experiment of HepG2 using 84880-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HeLa cells using NDUFS2 Recombinant antibody (84880-1-RR)

    FC experiment of HeLa using 84880-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84880-1-RR

    Affinity and Kinetic Characterization of 84880-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84880-1-RR against Human NDUFS2 were performed. The affinity constant is 1.61 nM.

NDUFS2 Recombinant monoclonal antibody

NDUFS2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84880-1-RR
Clone No.242238D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

242238D4, CI 49kD, CI-49kD, Complex I 49kD, Complex I-49kD

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84880-1-RR targets NDUFS2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag28008

Product name: Recombinant human NDUFS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-464 aa of BC000170

Sequence: GTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVEKLLNIRPPPRAQWIRVLFGEITRLLNHIMAVTTHALDLGAMTPFFWLFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLMDDIYQFSKNFSLRLDELEELLTNNRIWRNRTIDIGVVTAEEALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPVGSRGDCYDRYLCRVEEMRQSLRIIAQCLNKMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 53 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC000170
Gene Symbol NDUFS2
Gene ID (NCBI) 4720
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial Complex I, responsible for the oxidation of NADH, is suspected to be an integral component of the O2-sensing pathway. NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2), a key subunit of complex I in the mitochondrial electron transport chain (ETC), is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction (PMID: 30922174). Functions of NDUFS2 are associated with growth, cell-membrane integrity, ROS generation, apoptosis, necrosis, Complex I respiration, and synthesis of ATP (PMID: 33744462).

Protocols

Product Specific Protocols
FC protocol for NDUFS2 antibody 84880-1-RRDownload protocol
IHC protocol for NDUFS2 antibody 84880-1-RRDownload protocol
WB protocol for NDUFS2 antibody 84880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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