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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS1 Monoclonal antibody (68253-1-Ig)

    WB analysis using 68253-1-Ig (same clone as 68253-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NDUFS1 Monoclonal antibody (68253-1-Ig)

    WB analysis of HeLa using 68253-1-Ig (same clone as 68253-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IF Staining of HepG2 using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IF Staining of HeLa using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

NDUFS1 Monoclonal antibody, PBS Only

NDUFS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68253-1-PBS
Clone No.3H5E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68253-1-PBS targets NDUFS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3135

Product name: Recombinant human NDUFS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-305 aa of BC030833

Sequence: MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTAQPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEP

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 727 aa, 79 kDa
Observed molecular weight68-81 kDa
GenBank accession numberBC030833
Gene Symbol NDUFS1
Gene ID (NCBI) 4719
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP28331
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

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