Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12444-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12444-1-AP
WB result of NDUFS1 antibody (12444-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS1 transfected HEK-293 cells.
WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12444-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12444-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 12444-1-AP
IP result of anti-NDUFS1 (IP:12444-1-AP, 4ug; Detection:12444-1-AP 1:500) with mouse lung tissue lysate 2800ug.
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 12444-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 12444-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

NDUFS1 Polyclonal antibody

NDUFS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12444-1-AP

Publications(92)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (92)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse liver tissue, HEK-293 cells, A549 cells, HeLa cells, HEK-293 tissue, rat kidney tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 86 publications below
IHC	See 4 publications below
IF	See 5 publications below
CoIP	See 2 publications below

Product Information

12444-1-AP targets NDUFS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3135 Product name: Recombinant human NDUFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC030833 Sequence: MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTAQPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEP 相同性解析による交差性が予測される生物種
Full Name	NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Calculated molecular weight	727 aa, 79 kDa
Observed molecular weight	74-81 kDa
GenBank accession number	BC030833
Gene Symbol	NDUFS1
Gene ID (NCBI)	4719
RRID	AB_2282657
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28331
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Protocols

Product Specific Protocols
IF protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IHC protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IP protocol for NDUFS1 antibody 12444-1-AP	Download protocol
WB protocol for NDUFS1 antibody 12444-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res MFSD7c functions as a transporter of choline at the blood-brain barrier Authors - Xuan Thi Anh Nguyen View Article
human	WB	Cell Discov The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth Authors - Xiaojun Ren View Article
mouse	WB	Cell Metab Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals Authors - Timothy C Kenny View Article
mouse	WB	Nat Med Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Authors - Chouchani Edward T ET View Article
mouse	WB	Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. Authors - Xinxin Zuo View Article
mouse	WB	Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. Authors - Tingting Hou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12444-1-AP

WB analysis of mouse liver using 12444-1-AP

WB analysis of HEK-293 using 12444-1-AP

WB analysis using 12444-1-AP

WB analysis using 12444-1-AP

WB analysis of HEK-293 using 12444-1-AP

WB analysis of A549 using 12444-1-AP

IP experiment of mouse lung using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IF Staining of HepG2 using 12444-1-AP

IF Staining of HepG2 using 12444-1-AP

NDUFS1 Polyclonal antibody

NDUFS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MFSD7c functions as a transporter of choline at the blood-brain barrier

The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I.

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.