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NDUFS1 Polyclonal antibody

Name: NDUFS1 Polyclonal antibody
Brand: Proteintech
SKU: 12444-1-AP

NDUFS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12444-1-AP

Synonyms

CI 75Kd, Complex I 75kD, NDUFS1, PRO1304

Validation Data Gallery

Applications:

AllWBIPIHCIF

WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12444-1-AP
WB result of NDUFS1 antibody (12444-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS1 transfected HEK-293 cells.
WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12444-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12444-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12444-1-PBS
A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12444-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12444-1-PBS
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 12444-1-AP
IP result of anti-NDUFS1 (IP:12444-1-AP, 4ug; Detection:12444-1-AP 1:500) with mouse lung tissue lysate 2800ug.
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 12444-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 12444-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 12444-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse liver tissue, HEK-293 cells, A549 cells, HeLa cells, HEK-293 tissue, rat kidney tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)	IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 67 publications below
IHC	See 3 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

12444-1-AP targets NDUFS1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, zebrafish, Drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NDUFS1 fusion protein Ag3135 相同性解析による交差性が予測される生物種
Full Name	NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Calculated molecular weight	727 aa, 79 kDa
Observed molecular weight	74-81 kDa
GenBank accession number	BC030833
Gene symbol	NDUFS1
Gene ID (NCBI)	4719
RRID	AB_2282657
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Protocols

Product Specific Protocols
WB protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IHC protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IF protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IP protocol for NDUFS1 antibody 12444-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Discov The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth Authors - Xiaojun Ren View Article
mouse	WB	Cell Metab Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals Authors - Timothy C Kenny View Article
mouse	WB	Nat Med Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Authors - Chouchani Edward T ET View Article
mouse	WB	Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. Authors - Xinxin Zuo View Article
mouse	WB	Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. Authors - Tingting Hou View Article
human	WB	Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria Authors - Sabine Poerschke View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFS1 Polyclonal antibody

NDUFS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis using 12444-1-AP

WB analysis using 12444-1-AP

WB analysis of HEK-293 using 12444-1-AP

WB analysis using 12444-1-AP

WB analysis of mouse liver using 12444-1-AP

WB analysis of A549 using 12444-1-AP

WB analysis of A549 using 12444-1-PBS

WB analysis of HEK-293 using 12444-1-AP

WB analysis of mouse liver using 12444-1-PBS

IP experiment of mouse lung using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IHC staining of human liver cancer using 12444-1-AP

IF Staining of HepG2 using 12444-1-AP

IF Staining of HepG2 using 12444-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I.

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria