Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00097 targets NDUFB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 19-22 kDa |
| GenBank accession number | BC000466 |
| Gene Symbol | NDUFB8 |
| Gene ID (NCBI) | 4714 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | O95169 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NDUFS8 is encoded by a nuclear gene and is a member of the respiratory chain complex I. Respiratory chain complex I (CI; NADH: ubiquinone oxidoreductase), one of the largest membrane-bound protein complexes in the human cell, is essential for oxidative energy metabolism as it powers ATP synthesis by using the reducing potential of NADH to proton translocation across the inner mitochondrial membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NDUFB8 antibody RMX00097 | Download protocol |
| IHC protocol for NDUFB8 antibody RMX00097 | Download protocol |
| WB protocol for NDUFB8 antibody RMX00097 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |