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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using NDUFB4 Polyclonal antibody (27931-1-AP)

    WB analysis of mouse heart using 27931-1-AP (same clone as 27931-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27931-1-AP (NDUFB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27931-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NDUFB4 Polyclonal antibody (27931-1-AP)

    IHC staining of human stomach cancer using 27931-1-AP (same clone as 27931-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27931-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFB4 Polyclonal antibody (27931-1-AP)

    IF Staining of HeLa using 27931-1-AP (same clone as 27931-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFB4 antibody (27931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27931-1-PBS in a different storage buffer formulation.

NDUFB4 Polyclonal antibody, PBS Only

NDUFB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27931-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NADH-ubiquinone oxidoreductase B15 subunit, Complex I-B15, Complex I B15, CI-B15, CI B15

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27931-1-PBS targets NDUFB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27484

Product name: Recombinant human NDUFB4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-128 aa of BC000855

Sequence: MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMGALCGFGPLIFIYYIIKTERDRKEKLIQEGKLDRTFHLS

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC000855
Gene Symbol NDUFB4
Gene ID (NCBI) 4710
RRIDAB_2881012
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95168
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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