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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of human heart using 17614-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis using 17614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of mouse heart using 17614-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of rat heart using 17614-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of human heart using 17614-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of mouse spleen using 17614-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of mouse brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of mouse brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human ovary using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human ovary using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human heart using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human heart using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human kidney using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human kidney using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human skin using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human skin using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human liver using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human liver using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using NDUFB2 Polyclonal antibody (17614-1-AP)

    IF Staining of C2C12 using 17614-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using NDUFB2 antibody (17614-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NDUFB2 Polyclonal antibody

NDUFB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17614-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NADH-ubiquinone oxidoreductase AGGG subunit, Complex I-AGGG, Complex I AGGG, CI-AGGG, CI AGGG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, mouse spleen tissue, human heart tissue, mouse heart tissue, rat heart tissue, HuH-7 cells, MCF-7 cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse brain tissue, human heart tissue, human kidney tissue, human skin tissue, human liver tissue, human ovary tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17614-1-AP targets NDUFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB2 fusion protein Ag11789 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
Calculated molecular weight 95 aa, 11 kDa
Observed molecular weight 8-12 kDa
GenBank accession numberBC063026
Gene Symbol NDUFB2
Gene ID (NCBI) 4708
RRIDAB_2150944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.

Protocols

Product Specific Protocols
WB protocol for NDUFB2 antibody 17614-1-APDownload protocol
IHC protocol for NDUFB2 antibody 17614-1-APDownload protocol
IF protocol for NDUFB2 antibody 17614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Identification and validation of cuproptosis-related genes in acetaminophen-induced liver injury using bioinformatics analysis and machine learning

Authors - Zhenya Guo
View Article