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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFAF3 Polyclonal antibody (25621-1-AP)

    WB analysis using 25621-1-AP (same clone as 25621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25621-1-AP (NDUFAF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IHC staining of human ovary cancer using 25621-1-AP (same clone as 25621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25621-1-AP (NDUFAF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IF Staining of HeLa using 25621-1-AP (same clone as 25621-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFAF3 antibody (25621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

NDUFAF3 Polyclonal antibody, PBS Only

NDUFAF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NDUF3, E3 3, C3orf60, 2P1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25621-1-PBS targets NDUFAF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22514

Product name: Recombinant human NDUFAF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC002873

Sequence: MATALALRSLYRARPSLRCPPVELPWAPRRGHRLSPADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight17 kDa
GenBank accession numberBC002873
Gene Symbol NDUFAF3
Gene ID (NCBI) 25915
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU61
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404).

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