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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFAF3 Polyclonal antibody (25621-1-AP)

    WB analysis using 25621-1-AP (same clone as 25621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25621-1-AP (NDUFAF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IHC staining of human ovary cancer using 25621-1-AP (same clone as 25621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25621-1-AP (NDUFAF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFAF3 Polyclonal antibody (25621-1-AP)

    IF Staining of HeLa using 25621-1-AP (same clone as 25621-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFAF3 antibody (25621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

NDUFAF3 Polyclonal antibody, PBS Only

NDUFAF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NDUF3, E3 3, C3orf60, 2P1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25621-1-PBS targets NDUFAF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22514

Product name: Recombinant human NDUFAF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC002873

Sequence: MATALALRSLYRARPSLRCPPVELPWAPRRGHRLSPADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight17 kDa
GenBank accession numberBC002873
Gene Symbol NDUFAF3
Gene ID (NCBI) 25915
RRIDAB_3669488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU61
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404).

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