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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFAF1 Polyclonal antibody

NDUFAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15181-1-AP

Synonyms

CGI 65, CGI65, CIA30, NDUFAF1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 15181-1-AP

    WB analysis of HEK-293 using 15181-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 15181-1-AP

    WB analysis of Jurkat using 15181-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Transfected HEK-293 using 15181-1-AP

    WB analysis of Transfected HEK-293 using 15181-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15181-1-AP

    WB analysis of HeLa using 15181-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15181-1-AP

    IHC staining of human kidney using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with NDUFAF1 antibody included
  • IHC staining of human kidney using 15181-1-AP

    IHC staining of human kidney using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with NDUFAF1 antibody included

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, Transfected HEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDUFAF1 Ready-To-Use IHC Kit. NDUFAF1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

15181-1-AP targets NDUFAF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFAF1 fusion protein Ag7162 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1
Calculated molecular weight 38 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000780
Gene symbol NDUFAF1
Gene ID (NCBI) 51103
RRIDAB_2150778
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFAF1 antibody 15181-1-APDownload protocol
IHC protocol for NDUFAF1 antibody 15181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cell Res Ther

Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension.

Authors - Wenda Xi
View Article
mouseWB

Sci Rep

Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.

Authors - David W Reid
View Article