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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NDUFAF1 Polyclonal antibody (15181-1-AP)

    WB analysis of HEK-293 using 15181-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NDUFAF1 Polyclonal antibody (15181-1-AP)

    WB analysis of Jurkat using 15181-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using NDUFAF1 Polyclonal antibody (15181-1-AP)

    WB analysis of Transfected HEK-293 using 15181-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDUFAF1 Polyclonal antibody (15181-1-AP)

    WB analysis of HeLa using 15181-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using NDUFAF1 Polyclonal antibody (15181-1-AP)

    IHC staining of human oesophagus cancer using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using NDUFAF1 Polyclonal antibody (15181-1-AP)

    IHC staining of human oesophagus cancer using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using NDUFAF1 Polyclonal antibody (15181-1-AP)

    IHC staining of human oesophagus cancer using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NDUFAF1 Polyclonal antibody

NDUFAF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15181-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Complex I intermediate-associated protein 30, mitochondrial, CIA30, CGI-65, CGI65, CGI 65

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, Transfected HEK-293 cells, HeLa cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15181-1-AP targets NDUFAF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7162

Product name: Recombinant human NDUFAF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-327 aa of BC000780

Sequence: MALVHKLLRGTYFLRKFSKPTSALYPFLGIRFAEYSSSLQKPVASPGKASSQRKTEGDLQGDHQKEVALDITSSEEKPDVSFDKAIRDEAIYHFRLLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEVFLKMGKNNQSALLYGTLSSEAPQDGESTRSGYCAMISRIPRGAFERKMSYDWSQFNTLYLRVRGDGRPWMVNIKEDTDFFQRTNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNRGRIRDVQHELPLDKISSIGFTLADKVDGPFFLEIDFIGVFTDPAHTEEFAYENSPELNPRLFK

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1
Calculated molecular weight 38 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000780
Gene Symbol NDUFAF1
Gene ID (NCBI) 51103
RRIDAB_2150778
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y375
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for NDUFAF1 antibody 15181-1-APDownload protocol
WB protocol for NDUFAF1 antibody 15181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cell Res Ther

Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension.

Authors - Wenda Xi
View Article
mouseWB

Sci Rep

Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.

Authors - David W Reid
View Article
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